54I-3 |
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P.-W. LEE, J. A. Nordlee, and S. L. Hefle. Food Allergy Research and Resource Program, Univ. of Nebraska, 255 Food Industry Building, Lincoln, NE 68583-0919 Commercial ELISA methods are a powerful tool as part of a program of allergen control strategies for food manufacturers, and as an enforcement tool for regulatory agencies. Peanut is one of the most serious of the food allergens, responsible for most of the fatalities reported. AOAC Research Institute performance validated three commercial peanut kits last year, and FDA has indicated that they will use these for enforcement in the near future. However, the peanuts used in the AOAC study were of a certain type; given that many kinds of peanuts are used in foods, the ability of the kits to detect many types of peanuts needs to be evaluated. We examined three commercial peanut test kits (Neogen Veratox, r-Biopharm FAST, and Tepnel Biokit) to observe how different peanut varieties respond in them. Ten different peanut varieties were tested. Extraction and performance of the kits were according to each manufacturer's instructions. The ten peanut varieties used were mixed peanut (dry roasted) used for candy manufacture, Virginia NC-7 blanched and oil roasted, Spanish blanched, Spanish oil roasted, Flo-runner blanched, Flo-runner oil roasted, Georgia green dry roasted, mixed peanuts used for peanut butter dry roasted, mixed peanuts used for peanut butter dry roasted with skins, and a dry roasted high oleic variety. In general, blanched peanuts registered higher concentrations than did roasted. Peanut butter mixes with and without skins registered the same values in each kit. Georgia green peanuts registered close values between kits (173,000 to 175,000 ppm), but other varieties were much more disparate, with values ranging from 20,183 to > 1.6 million ppm. Recognition in commercial kits is due to antibody specificity and peanut varieties used in standard preparation. When standards are made and validations performed, as many peanut varieties as possible should be included. Evaluating variety differences is important when using peanut ELISA systems.
Session 54I, Toxicology & Safety Evaluation: General
2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana |