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N. T. DUNFORD, Dept. of Plant & Soil Sciences, Oklahoma State Univ., 103 FAPC, Stillwater, OK 74078, S. Irmak, Food and Agricultural Products Research and Technology Center, Oklahoma State Univ., FAPC 103, Stillwater, OK 74078, J. Trad, Plant and Soil Sciences, Oklahoma State Univ., FAPC 103, Stillwater, OK 74078, S. E. Gilliland, Dept. of Animal Science, Oklahoma State Univ., 101 Animal Science Bldg., Stillwater, OK 74078-6051, and H. B. Dunford, Dept. of Chemistry, Univ. of Alberta, Edmonton, Alberta, Canada. Conjugated linoleic acid (CLA) refers to a group of geometrical and positional isomers of linoleic acid (LA) with conjugated double bonds. CLA has been reported to have diverse health benefits and biological properties. Although conversion of LA to CLA by microorganisms has been reported extensively, literature on the utilization of enzyme extracts and purified enzyme preparations for LA isomerization is limited. The objective of this study was to develop a process for biocatalysis of LA to CLA. Crude enzyme extracts were prepared from Lactobacillus acidophilus L1 (obtained from the stock culture collection of the Food Microbiology Laboratory at the Food and Agricultural Products Research and Technology Center, Oklahoma State University), and 2 strains of Lactobacillus reuteri (ATCC 23272 and ATCC 55739). Bioconversion of free LA to CLA was carried out at 20, 37 and 50oC for 3, 24 30 h using enzyme extracts. The composition of reaction products was analyzed using GC/MS. All 3 cultures used in this study were able to isomerase LA. However, the enzyme assays carried out on crude extracts indicate significant differences in specific activity, initial LA to CLA conversion rates and the reaction products for the 3 different sources of enzyme.
Session 17, Biotechnology: General
2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana |