89D-4


Anti-Listerial activities of soy protein edible film coating containing grape seed extract (GSE), Nisin and EDTA and their combinations on full fat turkey frankfurters stored at 4°C and 10°C

T. SIVAROOBAN1, N. S. Hettiarachchy1, B. Adams1, and M. G. Johnson. (1) Dept. of Food Science, Univ. of Arkansas, 2650, N. Young Ave, Fayetteville, AR 72704

Food-borne pathogens are a great concern to consumers and are responsible for annual significant losses to the food industry. Listeria monocytogenes (L. monocytogenes) is among the most important pathogens that have caused a significant number of outbreaks. Finding potent natural sources for controlling L. monocytogenes is necessary to minimize pathogen contamination concerns.

The objective of this study is to evaluate the inhibitory effect of soy protein film coating containing grape seed extract (GSE) with and without nisin and / EDTA coated onto full fat turkey frankfurters against L. monocytogenes stored at 4 oC or 10 oC for 60d.

Testing has been conducted by surface inoculation of activated L. monocytogenes (strain V7, serotype 1/2a) onto 1 g pieces of full fat turkey frankfurters and dipped into soy protein film forming solution with/without the addition of antimicrobial agents (GSE/Nisin/EDTA/combinations), and stored at 4 oC or 10 oC. The antimicrobial activity of coating was evaluated on a weekly basis for 60 d. The highest inhibitory activity was found in the sample containing GSE combined with nisin and EDTA. After 28 d at 4 oC the growth of L. monocytogenes (7.3 CFU/g) was suppressed to undetectable level in the soy protein film coating containing GSE combined with nisin and EDTA (reductions of 7.0 to 7.5 logs CFU/g). However, the samples stored at 10 oC did not show any significant reductions on the growth of L. monocytogenes.

The soy protein film coating containing GSE, nisin and EDTA is effective in inhibiting L. monocytogenes growth at 4 oC. This finding will have applications in food products including raw and ready-to-eat poultry and beef products, as well as fresh whole and minimally processed vegetables and fruits for controlling L. monocytogenes contamination.

Session 89D, Food Microbiology: Antimicrobial effects on foodborne microorganisms
2:00 PM - 5:30 PM, Tuesday PM Room Hall I-2

2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana