89A-27

A. C. M. OLIVEIRA¹, K. Brenner¹, J. Chantarachoti¹, C. Vorholt¹, P. J. Bechtel², and C. A. Crapo¹. (1) Fishery Industrial Technology Center, Univ. of Alaska, Fairbanks, School of Fisheries & Ocean Sciences, 118 Trident Way, Kodiak, AK 99615-7401, (2) Subarctic Agricultural Research Unit, USDA-ARS-Pacific West Area, Univ. of Alaska, Fairbanks, 245 O'Neill Bldg., Fairbanks, AK 99775-7220

Accelerated solvent extraction (ASE) systems use solvents at high temperatures and pressures to minimize of solvent volume and extraction time. The system is fully automated and extraction takes place under nitrogen.The objective of this research was to compare lipid recoveries from fish muscle samples using the ASE200 and the extraction method described by Folch. Alaska pink salmon fillets from six fish were mixed and comminuted to paste then subdivided into four sub-samples. Cod liver oil (CLO) was added to three sub-samples at levels of 2%, 4%, or 6% of the total sample weight. Lipids were extracted in triplicate from each subsample and lipid level. The ASE200 (Dionex, Sunnyvale, CA) was operated at 80⁰C under 1500 psi for a total time of 15 min using dichloromethane (DM) or a 2:1 mixture of chloroform/methanol (CM). Lipids were quantified gravimetrically and lipid recoveries statistically compared. Lipid content of salmon fillets using Folch, ASE with DM, and ASE with CM were not different (p<0.05) at 2.4%, 2.3%, and 2.6%, respectively. Lipid recoveries using Folch methodology for samples with 2%, 4% and 6% of CLO added were not significantly different averaging 88%, 90.5%, and 86%, respectively. Lipid recoveries determined with ASE using either solvent system were not significantly different than Folch methodology for comparable CLO samples. Recoveries using ASE with DM were not different (p<0.05) and averaged 80.6%, 81.9% and 83.6% for the 2%, 4%, and 6% CLO samples, respectively. Recoveries using ASE with DM were not different (p<0.05) for samples with 2%, 4%, and 6%CLO added and averaged 83.4%, 83.3% and 85.4%, respectively. Results suggest that it is possible to use an ASE system for determination of lipid content in fish samples. Further investigations using the ASE200 will include longer extraction times and multiple extraction cycles to achieve 95% lipid recoveries.

Session 89A, Aquatic Food Products: General
2:00 PM - 5:30 PM, Tuesday PM Room Hall I-2

2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana