36B-20 |
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A. R. P. Scamparini1, A. L. Berwanger2, N. Domingues2, L. T. Vanzo2, H. Treichel2, and F. F. PADILHA2. (1) Food Science, UNICAMP, Monteiro Lobato, 80, Campinas/SP, Brazil, (2) Food Engineering, URI - Campus de Erechim, Av. Sete de Setembro, 1621, Erechim/RS, Brazil Glucose and sucrose are generally used as preferential carbon sources for the production of biopolymers. Nevertheless, alternative sources such as molasses, soybean pomace, cheese whey and others, have been suggested, aiming at the reduction of production costs. The main objective of this work was the production and rheological characterization of biopolymer by Sphingomonas capsulata ATCC 14666, using conventional and industrial media in rotary shaker at 28ºC and 72h. The production of biopolymer in conventional medium was carried out using a full 22 factorial design with 3 replicates at the center point to establish the optimum conditions of each independent variable (concentration of sucrose and agitation). The productivity reached the maximum of about 0.038 g/Lh, at 208 rpm and 4% (wt/v) of sucrose. For this optimum condition, different concentrations of industrial medium was tested (2.66, 4, 6, and 8%, wt/v). Tukey's test was used to check significant differences among conditions studied. The best productivities was obtained using pretreated molasses 8% (wt/v) (0.296 g/Lh), residue of TSP 6% (wt/v) (0.244 g/Lh) and crude molasses 8% (wt/v) (0.192 g/Lh), respectively. The rheology (apparent viscosity) presented similar values compared to those of the literature for other biopolymers. This project is very important for regional desenvolviment.
Session 36B, Biotechnology: General
2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana |