83-8 |
|
S. K. YOO, Food and Biotechnology and Genseng Medicinal Herbs Bio-Food Research Center, Joongbu Univ ., Geumsan-Gun, Choongchungnam-Do, 312-702, South Korea, Y.-S. Chang, Food Science and Biotechnology, Joongbu Univ ., South Korea, and M.-S. Kim, Food Science and Biotechnology, Joongbu Univ., South Korea. The method development of the separation and quantification ginsenosides in ginseng may supply a variety of application to functional foods, cosmetics, and fine chemical industries. Most of gensenosides have been analyzed by HPLC. However, the separation and quantification by HPLC have many limitations and drawbacks due to the similar structure of ginsenosides. The objective of this study was to develop a rapid method for separation and quantification of ginsenosides in ginseng extracts. For extraction of ginsenosides from ginseng, 1 g of dried ginseng was soaked in 10 ml butanol saturated with water, stored at – 4oC for 24 h, sonicated for 30 min, and centrifuged at 1500 rpm for 5 min. The supernatant was collected and vacuum- evaporated to dryness at 60oC. The extract was dissolved in 1 ml methanol. For analysis of the ginsenosides , TLC silica gel 60 F 254 (Merck) was used. The solvents were chloroform, metanol, and water (65: 35: 10). Two ascents were conducted. The plates were air- dried, and the spots were visualized with dipping in 5% sulfuric acid. The plates were heated at 100oC for 10 min. Densitometry and image analyzer performed quantification of ginsenosides. Rb,Rc, Rd, Re, Rf, and Rg1 were used as a standard. The rhizome consisted of 0.97 mg/g Rb, 0.85 mg/g Rc, 0.87 mg/g Rd, 0.70 mg/g Re, 0.61 mg/g Rf, and 0.62 mg/g Rg1. The lateral root consisted of 0.73 mg/g Rb, 0.76 mg/g Rc, 0.79 mg/g Rd, 0.80 mg/g Re, 0.71 mg/g Rf, and 0.68 mg/g Rg1. The hairy root consisted of 0.82 mg/g Rb, 0.84 mg/g Rc, 0.81 mg/g Rd, 0.72 mg/g Re, 0.74 mg/g Rf, and 0.76 mg/g Rg1. The application of this method could be applied to need a rapid evaluation of ginsenosides in ginseng products industry.
Session 83, Food Chemistry: Antioxidant and bioactive agents
2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana |