18E-24 |
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S. J. KIM1, Y. S. Oh2, N. Y. Park, J. Y. Shin, S. H. Lee, and K. S. Youn. (1) Dept. of Food Science and Technology, Catholic Univ. of Daegu, Hayang-eup, Gyeongsan-si, 712-702, South Korea, (2) Institute of Biotechnology, Yeongnam Univ., Dae-dong, Gyeongsan-si, South Korea Obesity is known to be closely related to diet, exercise, and genetic factors, although its exact mechanism is not fully understood. Many studies are focusing on the investigation of obesity mechanism and the development of obesity-suppressing agents, especially those with no harmful effects. In our study, we have studied obesity-suppressing foodstuffs and identified a co-supplement of whey extracts that show synergism with inhibitory capacity of plant extracts. This study was designed to investigate the effect of co-supplement of whey extracts with rose petal and Schisandra chinensis fruit extracts on lipid accumulation and differentiation of 3T3-L1 cells within a patient. Whey extracts of various concentrations were mixed with rose petal or Schisandra chinensis fruit extracts and administered to 3T3-L1 preadipocytes at the outset of the differentiation program and further incubated for 10 days. Cells were maintained in post differentiation medium containing insulin with each extract in complete culture medium. Morphology, triglyceride concentration and glycerol-3-phosphate dehydrogenase activity was compared 10 days after incubation. Rose petal and Schisandra chinensis fruit extracts inhibited the lipid accumulation and adipocyte differentiation of 3T3-L1 preadipocytes in a dose-dependent manner. Whey extracts prepared from milk and yogurt also inhibited lipid accumulation and preadipocytes, although it was less effective than plant extracts. When whey extracts were co-administered with plant extracts, the accumulation of triglyceride in adipocytes was suppressed compared to that of plant extract alone. The change of glycerol-3-phosphate dehydrogenase activity showed a similar trend as that of triglyceride content. Supplement of milk whey extract was more effective than with yogurt. These results suggest that rose petal and Schisandra chinensis fruit could be good candidates as anti-obesity foodstuffs, as could whey extracts. In addition, it is possible to improve the anti-obesity capacity of plant extracts by co-supplementation with whey extracts.
Session 18E, Nutraceutical & Functional Foods: General I
2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana |