36B-17 |
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S. K. YOO, Food and Biotechnology and Genseng Medicinal Herbs Bio-Food Research Center, Joongbu Univ., Geumsan-Gun, Choongchungnam-Do, 312-702, South Korea, Y. S. Chang, Department o food and biotechnology and Genseng Medicinal Herbs Bio-Food Research Center, Joongbu Univ., South Korea, and M.-S. Kim, Food Science and Biotechnology, Joongbu Univ., South Korea. Mannitol as a GRAS can be used as a food additive; anticaking, stabilizing, thickening, and texturizing and as medical treatments and as an antioxidant for reducing plasma hydrogen peroxide. The objective of this study was to investigate the conversion of fructose to mannitol in a packed bed reactor in semi-continuous mode bacterial cells immobilized on the activated carbons. A new strain producing mannitol from fructose was isolated from the broth of kimchi and designated as Leuconostoc sp. strain JY1. The immobilized reactor, a 250 x 60 mm glass column with water-cooling jacket, was packed with activated carbons (30 x 80 mesh) and connected to a peristaltic pump. Fructose (5%, w/v) was pumped with a downward flow of 5 ml/min and the outlet stream was continuously recalculated into a reservoir and back to the column. Five consecutive batches using fresh medium for each one were carried out at 25oC. No column washing procedure was performed between each treatment batch. The mannitol and fructose were assayed by TLC. As the number of batches increased, the conversion yield was decreased. The highest yield (0.82 g/g) and productivity (0.56 g/l-h) were obtained in the first batch. An increased number of cycles resulted in better system performance due to cell adaptation to the medium. This procedure offers economical production of mannitol from fructose.
Session 36B, Biotechnology: General
2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana |