18E-16 |
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R. DÍAZ-SOBAC, Instituto de Ciencias Básicas-Fac. Química Farmacéutica Biológica, Univ. Veracruzana, Dr. R. Sánchez Altamirano s/n. Colonia Industrial Animas, Calle de la Pérgola s/n Zona Universitaria, Xalapa, Veracruz, 91192, Mexico and A. Martínez-Cabrera, Maestria en Ciencias Alimentarias, Univ. Veracruzana, Apdo. Postal 575, Xalapa, Mexico. Spirulina platensis is a microalga that synthesizes a great variety of chemical compounds as to ficobiliprotein phycocyanin . Prior studies indicate that is capable of inhibiting processes of lipid peroxidation capturing free radicals. It is probable that the phycocyanin can induce inhibition in processes related to the phenomenon of stress oxidative, capturing radicals superoxide (O2*). The objective of this work was to evaluate the protective effect in processes of stress oxidative, that the phycocyanin has in a model system of blood serum. The isolation of phycocyanin was carried out for centrifugation using ammonia sulphate. Subsequently, the extracts were freeze-dried. The identification and quantification was carried out for spectrophotometric methods and electrophoresis . The inhibition of the production of the (O2*) I am determined for the method of autooxidation of the pyrogallol. The results show that in the autooxidation of pyrogallol, to concentrations of 15 and 30 mM were where the collecting of (O2*) was greater in a 72.4% to concentration of 100 ƒÝg/ml of phycocyanin. These results suggest that the phycocyanin capture (O2*) in processes of oxidation of pyrogallol, what possibly the mechanism consist of inhibiting the steps of formation and/or propagation of (O2*), which they are important so that the processes of oxidation be carry out. This result suggests that this composed can have an activity biofunctional important in against the processes of stress oxidative.
Session 18E, Nutraceutical & Functional Foods: General I
2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana |