18E-10 |
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Y.-S. WONG and W.-S. Lau. Biology Dept., The Chinese Univ. of Hong Kong, Shatin, N.T., Hong Kong SAR, China Epidemiological studies support a prominent role of dietary flavonoids in preventing many chronic diseases such as cancer and cardiovascular disease. Recent studies showed that anthocyanins inhibited tumor development in experimental animals. The objective of this study was to investigate the anti-proliferative effects of cyanidin and cyanidin-3-O-glucoside, two major anthocyanins present in many fruits and vegetables, on two human cancer cell lines including human liver cancer HepG2 cell line and human breast cancer MCF-7 cell line. The 5-bromo-2'-deoxyuridine (BrdU) incorporation assay was used to assess inhibition of cell proliferation and the flow cytometry was employed to study the mode of action of cell growth inhibition. Our results showed that both cyanidin and cyanidin-3-O-glucoside exhibited anti-proliferative activities on HepG2 and MCF-7 cell lines in a dose-dependent manner. The cell growth inhibitory effect of cyanidin was higher than that of its glucoside. The IC50 values of cyanidin against HepG2 and MCF-7 were 72.2 and 129 μM respec-tively. No cytotoxic effect of these two compounds was observed on normal human cell line Hs68. Flow cyto-metry analyses suggested different modes of cell cycle impairment occurred in the two cancer cell lines treated with cyanidin. These findings support the potential health-beneficial properties of dietary anthocyanins.
Session 18E, Nutraceutical & Functional Foods: General I
2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana |