36B-9 |
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H.-S. Song1, J.-H. Kim1, S.-H. Park2, and H.-Y. KIM1. (1) Department of Food Science and Biotechnology, Kyung Hee University, Suwon, 449-701, South Korea, (2) Division of Nutrition Evaluation, Korea Food and Drug Administration, Seoul, 122-704, South Korea The GMO (Genetically Modified Organism) labeling system on raw materials has been enforced in Korea since March 2001, and GMOs derived foods since July 2001 and is performed by displaying five kinds of GMOs, such as soybean, maize, potato, canola and cotton. The development of a practical detection method is required to confirm the validity of labeling system and to monitor the status of circulation for GMO. Among the detection methods, multiplex PCR-based method is that several target DNA sequences can be screened for and detected in a single reaction. It is most suitable for the purpose of screening because of simplicity and economy. Our objective was to design the multiplex PCR method to ascertain the validity of labeling system and to monitor the status of circulation for GM Maize. The eight lines of GM Maize (Bt11, T25, Mon810, Event176, Mon863, TC1507, GA21 and NK603) were used and specific primer pairs from cry1Ab, pat, promoters and terminators inserted in the different lines of GM Maize were designed to detect each line. Our results showed the different lines of GM Maize were monitored from raw products and processed foods in Korean market using multiplex PCR method. Some of the maize processed foods and raw materials were shown to contain more than one foreign gene. These results suggest that multiplex PCR method would be effective for detecting eight different GM Maize in a single reaction and apply to screen GM Maize in the market.
Session 36B, Biotechnology: General
2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana |