18B-9 |
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Z. WANG, School of Food Science and Technology, Southern Yangtze Univ., 170 Huihe Road, P.B 98, Wuxi, 214036, China and S. Xu. Lily is widely cultivated in China for food and ornamental use. Non-starch polysaccharides (NSP) were extracted and studied because NSP are important in the normal digestive and absorptive function of the gut, especially the large intestine, and they protect human from some diseases. Our objective was to extract, isolate and purify the water soluble non-starch polysaccharides (WSNSP) from fresh lily and to investigate the biological activity of them. Water soluble non-starch polysaccharides (WSNSP) were extracted from fresh lily bulb using water. Protein was removed by a non-protease method, that is, the Sevaga method. DEAE-Sepharose-CL-6B and Sepharose CL-6B chromatography were used to separate WSNSP into four fractions.The chemical structure of the pure WSNSP was determined by various analytical methods (GC, IR, Periodate Oxidation, Smith degradation, Methylation, 13C-NMR analysis.).From high performance gel filtration chromatography determination ,the molecular weighs of the four fractions A,B,C and D were 216, 11.1, 268, and 92.1kDa,respectively. No protein was detected in fractions A and B, while fractions C and D contained some protein. This indicated that fraction C and D were glycoproteins, that is ,the carbohydrates and proteins parts were chemically combined together. Fraction B was determined to have a backbone composed of ¦Á-D(1,4)-Glu and b-D(1,3)-Man(1:1.7) and a short ¦Á-D(1,6)-Glu branch, attached to the main chain at position 6. The WSNSP from lily bulb of the fraction B directly inhibited the growth of tumor cell, while in vivo tests revealed that the WSNSP of the fraction B had an anticancer function, being able to restrain the S180 growth (45.68%) of mice.
Session 18B, Food Chemistry: Antioxidant and bioactive agents
2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana |