54I-8 |
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M. -F. Wen1, K. -S. Chang2, C. -L. Hsu2, and H. -J. HOU3. (1) Dept. of Food Science, Yuanpei Univ. of Science & Technology, 306, Yuanpei Street, Hsinchu, 300, Taiwan, (2) Dept. of Food Science, Yuanpei Univ. of Science and Technology, 306 Yuanpei St., Hsinchu, 300, Taiwan, (3) Research & Development, White Wave, INC, 1990 N. 57th Court, Boulder, CO 80301 Hydrogen peroxide is commonly used as a food preservative in milk and cheeses or as a sterilant of packaging materials due to its inherent sporicidal and bactericidal properties. Detection and quantification of hydrogen peroxide in the food industry often involves spectrometric, fluorescence or chemiluminescence assays which employ horseradish peroxidase (HRP) and follow the oxidation of different commercial substrates in the presence of Hydrogen peroxide. This project developed a detecting system, using a palladium chemically modified electrode, which can be applied in quantitatively determination of hydrogen peroxide residue in an aseptic package which is usually sterilized using hydrogen peroxide during processing. Amperometric measurements were performed using a laboratory-built potentiostat. For the amperometric measurement of hydrogen peroxide, 1 mL of hydrogen peroxide solution of various concentrations was injected into the test solution using a micro syringe. The response current was displayed and simultaneously logged by the computer. The difference between the baseline and the steady-state current was used to calculate the concentration of hydrogen peroxide. The Pd-modified strip electrode exhibited an anodic response to hydrogen peroxide starting at +0.2 V, reaching its diffusion current limit at about +0.4 V. The anodic current produce of hydrogen peroxide for the Pd deposited carbon strip electrode increased with increasing in voltage from +0.1 to +0.7 V. The carbon strip electrode produced a current response about 30 fold smaller than that of Pd strip. The sensitivity of Pd electro-deposition electrodes was improved 30-fold compared with the unmodified electrode, resulting in an increase in surface activity that can be used for the detection of the hydrogen peroxide liberated from enzymatic reactions. Thus, the Pd electro-deposition was found effective on improving the amperometric behavior of the thick-film carbon electrodes. The sensor can be used to detect the hydrogen peroxide residue in aseptic package.
Session 54I, Toxicology & Safety Evaluation: General
2005 IFT Annual Meeting, July 15-20 - New Orleans, Louisiana |