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S. JONES1, R. R. Bansode1, H. A. Bawadi2, J. W. Bell1, M. W. Moody1, and J. N. Losso1. (1) Dept. of Food Science, Louisiana State Univ. Agricultural Center, 111 Food Science Bldg., Baton Rouge, LA 70803-4200, (2) School of Human Ecology, Louisiana State Univ. Agricultural Center, 125 Human Ecology Bldg., Baton Rouge, LA 70803 Angiogenesis is associated with diseases such as obesity, diabetes complications, arthritis, atherosclerosis, and cancer. Most angiogenic diseases share the same stimulators. Most synthetic drugs that inhibit angiogenesis in animal models are not approved for humans because of toxicity or lack of efficacy. There is a growing interest and evidence in the role of dietary compounds (functional foods) as inhibitors of early stages of angiogenesis. The literature is well established that shark cartilage and bovine collagen peptides are promising low budget prescription for several angiogenic diseases. However, the outbreak of bovine spongiform encephalopathy (BSE) also known as mad cow disease and the need for “viral free-products are suggesting that more efficient healthcare products will originate from the sea. The objective of this study was to evaluate the potential anti-angiogenic activities of peptides generated by enzymatic hydrolysis of black drum skin or alligator cartilage collagen. Black drum and alligator cartilage collagen were hydrolyzed with acid protease and the peptides were recovered by gel filtration. The molecular weight of the hydrolyzates was obtained using MALDI-TOF-MS. Black drum and alligator collagen hydrolyzates were incubated with normal human lung fibroblast and colon cancer Caco-2 cells in serum-free medium for 24 h. Angiogenesis was evaluated by cell proliferation and migration assays. Inhibition of metalloproteinase-9 (MMP-9) was estimated by ELISA. Cell cytotoxicity was assessed by luminescence. Black drum and alligator collagen peptides ranged up to 1923 and 2205 daltons, respectively. At 2 microM, peptides from black drum and alligator cartilage collagen significantly inhibited cell proliferation, weakly prevented cell migration in the Boyden Chamber, and did not cause cell death. But both peptides inhibited MMP-9 (angiogenesis stimulator) activities in the extracellular medium. Collagen peptides from aquatic food products are good substitutes for land-based animal collagen peptides and will create better economic opportunities for seafood processors.
Session 63, Aquatic Food Products: Quality, processing, antioxidants and surimi
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