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S. DATTA1, R. R. Bansode1, H. A. Bawadi2, and J. N. Losso1. (1) Dept. of Food Science, Louisiana State Univ. Agricultural Center, 111 Food Science Bldg., Baton Rouge, LA 70803-4200, (2) School of Human Ecology, Louisiana State Univ. Agricultural Center, 125 Human Ecology Bldg., Baton Rouge, LA 70803 Glycomacropeptide (GMP), the poplypeptide fraction containing the 106-169 fragment of kappa-casein as a result of chymosin hydrolysis is high in proline and has been recognized for health enhancing properties such as antiviral, antimicrobial, modulation of the immune system, and appetite suppression. We hypothesized that glycomacropetide hydrolyzates may have inhibitory effect against the process of angiogenesis. The objective of this study was to evaluate the potential inhibitory activity of GMP peptides against cancer cell proliferation and migration using an in vitro model of angiogenesis. Bovine and ovine GMP were sequentially hydrolyzed by pepsin and pancreatin and peptides less than 5,000 daltons were recovered by gel filtration. The molecular weight profile of the peptides was obtained by using MALDI-TOF-MS. Normal human lung fibroblast cells and Caco-2 cells were starved in serum-free medium and incubated for 24 h with 0- 1 microM. Cell proliferation was evaluated by measuring the bioluminescent ATP present in metabolically active cells. Cell migration assay was carried using a QCM 96-well Chemotaxis Migration assay. Both GMP peptides at 1 microM did not affect the proliferation of normal cells. Both GMP peptides at 1 microM significantly inhibited Caco-2 cell proliferation. Bovine GMP peptides at 1 microM did not prevent cell migration to the FBS chemoattractant in the lower chamber of the Chemotaxis system but significantly slowed the growth of cancer cells in 24 h. Goat GMP peptides at 1 microM caused cell death after 24 h of incubation and reduced cell migration to the chemoattractant containing chamber. The results suggest additional activities for bovine and ovine (goat) GMP peptides that include the inhibition of cancer cell proliferation.
Session 46, Dairy Foods: General
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