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H. A. BAWADI1, R. R. Bansode2, J. N. Losso2, and A. F. Trappey, II2. (1) School of Human Ecology, Louisiana State Univ. Agricultural Center, 125 Human Ecology Bldg., Baton Rouge, LA 70803, (2) Dept. of Food Science, Louisiana State Univ. Agricultural Center, 111 Food Science Bldg., Baton Rouge, LA 70803-4200 Wild muscadine (Vitis rotundifolia Michx.) is found in Louisiana, the Gulf of Mexico, Delaware, Georgia, and many Midwestern states. The grapes are known for their content of valuable phenolic compounds such as the anthocyanins, the proanthocyanidins, and resveratrol which all impart the health enhancing properties ascribed to muscadine juice. Improved recovery during reperfusion after ischemia was reported in rats fed grape proanthocyanidins extracts. Angiogenesis is the hallmark of cancer and various ischemic and inflammatory diseases. We hypothesized that muscadine proanthocyanidins may also have anti-angiogenic effects against cancer proliferation. The objective of this study was to determine the molecular weight profile of oligomeric proanthocyanidins in wild muscadine and evaluate their potential against cancer cell proliferation, migration, and the secretion of the major cytokine vascular endothelial growth factor (VEGF165). The molecule weight profile of the proanthocyanidnis was obtained using MALDI-TOF-MS. Caco-2 and MCF-7 breast cancer cells and normal human lung fibroblast cells were incubated for 24 h in serum-free media with 0-50 micromoles of muscadine proanthocyanidins. In vitro angiogenesis assays include the HUVEC tube formation assay, the bioluminescent measurement of ATP present in cell after treatment with proanthocyanidins, the QCM Chemotaxis 96-well cell migration assay, and the levels of soluble VEGF165. Results indicated that (1) the oligomeric proanthocyanidins were mostly trimers and tetramers (m/z between 875 and 1277) and (2) at 50 micromoles did not interfere with normal human cell proliferation. However, proanthocyanidins dose-dependently inhibited tube formation (IC50: 34 micromoles); decreased cell proliferation; and at 50 micromoles prevented the migration of cancer cells in the presence of 10% FBS as chemoattractant. Proanthocyanidin-treated cancer cells induced low levels of VEGF165 receptor expression (< 35% of the control). Wild muscadine proanthocyanidins inhibited angiogenesis in vitro and in vivo assay and strategies to enhance their bioavailability should be sought.
Session 29, Food Chemistry: Antioxidants and bioactive agents
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