114E-23 |
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F. G. LOARCA-PIÑA1, V. Olvera-García2, R. Reynoso-Camacho2, G. Elizondo-Azuela3, E. Castaño-Tostado1, and O. Mora-Izaguirre4. (1) Programa de Posgrado en Alimentos del Centro de la República, Universidad Autónoma de Querétaro. Facultad de Química, Centro Universitario, Cerro de las Campanas S/N, Querétaro, 76010, Mexico, (2) Programa de Posgrado en Alimentos del Centro de la República, Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas S/N, Querétaro, 76010, Mexico, (3) Programa de Posgrado, Sección Externa de Toxicología, CINVESTAV/Instituto Politécnico Nacional, Av. Instituto Politécnico Nacional 2508, Colonia San Pedro Zacatenco, Distrito Federal, 07360, Mexico, (4) Laboratorio de Rumiología y Metabolismo Nutricional, Facultad de Estudios Superiores-Cuautitlán/UNAM, Juriquilla, Apartado Postal 1-1141, Querétaro, 76230, Mexico In Mexico, calyxes from Jamaica flower (Roselle) are consumed as a fresh beverage and sometimes with a medical purpose due to its biological properties. Roselle calyxes are rich in phenolic compounds and have been reported their antioxidant, antimutagenic and probably anticarcinogenic activities. On the other hand, cancer is one of the most important chronic-degenerative diseases in the world; in Mexico cervical and breast cancer are the most frequent cancer types among female population. The objective of this study was to evaluate the possible nutraceutical effect of aqueous extract of H. sabdariffa L by its capacity to decrease viability of HeLa cells and suggest a possible mechanism of action of this extract. The amount of total phenolic compounds in the aqueous extract was determinate using the Folin-Ciocalteau method (25.04 ± 2.1 mg equivalents of protocatechuic acid/g lyophilized extract). Effect of aqueous extract was tested on viability of HeLa cells using the Metil Tetrazolium assay. Cell viability obtained at 24 hours was 68, 55 and 7% for 5, 7.5 and 10 mg/mL, respectively. Concentration of 7.5 mg/mL and 24 hours were chosen to evaluate the effect of extract on DNA fragmentation. The expressions of proteins Bax, Bcl-2 and Caspase-3 in treated HeLa cells were evaluated using western blot. Results showed that aqueous extract is not capable of induce DNA fragmentation, and that the protein expressions decrease at 24 hours. These results suggest that treated cell death is not due to apoptosis.
Session 114E, Nutraceuticals & Functional Foods: Bioactivity measurement and mechanism
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