49I-16 |
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X. WU1, J. S. Smith1, and J. F. Leslie2. (1) Food Science Institute, Kansas State University, 230 Call Hall, Manhattan, KS 66506, (2) Plant Pathology, Kansas State University, 4002 Throckmorton Plant Sciences Center, Manhattan, KS 66506 Fusaproliferin is a mycotoxin produced by several Fusarium species commonly found on cereal grains. It has been shown to be toxic to Artemia salina, insect and human cell lines, and teratogenic to chicken embryos. One recent study showed that fusaproliferin can form adducts with DNA fragments suggesting its interactions with DNA and possible mutagenicity. Our objective was to evaluate the mutagenic potential of fusaproliferin using the Ames Salmonella/microsome assay. The standard plate-incorporation method was employed to evaluate the mutagenic potential of fusaproliferin in five S. typhimurium tester strains (TA1535, TA100, TA98, TA97a, and TA102). Each strain was evaluated with five levels of fusaproliferin ranging from 1 to 100 mg/plate. All the tests were performed in duplicates with blank, solvent(DMSO), and positive controls. Aflatoxin B1, 2-aminofluorene, and 2-aminoanthracene served as positive controls for all the strains when rat liver S9 was added (from Aroclor-1254 induced Sprague Dawley males). NaN3 was the positive control for TA1535 and TA100, and Dexon was the positive control for TA98, TA97a, and TA102 when no S9 was added. Revertants were hand counted after 72 hour incubation at 37 °C. All the experiments were repeated three times. Fusaproliferin did not show any mutagenicity in TA1535, TA100, TA97a, and TA102, whereas, it did exhibit mutagenic activity in TA98 with added S9. The mutagenic potency of fusaproliferin in TA98 was much weaker than that of aflatoxin B1 (2.4 revertants/mg of fusaproliferin vs 950 revertants/mg of aflatoxin B1). Our results suggest that fusaproliferin with S9 activation is a weak mutagen in S. typhimurium TA98. This is the first report about mutagenicity of fusaproliferin. More research work is needed to evaluate the nature of its mutagenic effect and what hazards it may present to human and animal health.
Session 49I, Toxicology & Safety Evaluation: General
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