67C-7 |
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P. GADGIL1, K. A. Hachmeister2, D. H. Kropf2, and J. S. Smith1. (1) Food Science Institute, Kansas State University, 229 Call Hall, Manhattan, KS 66506, (2) Animal Sciences and Industry, Kansas State University, 201 Weber Hall, Manhattan, KS 66506 Alkylcyclobutanones (ACB’s) are radiolytic products formed when fatty acids are irradiated. These cyclobutanones are unique irradiation byproducts and therefore may serve as indicators of irradiation exposure. There is limited data available about ACB formation in retail meat products. Therefore, reliable methods are desirable which can quantify ACB’s and thus estimate the irradiation dose in commercially available meat products. The cyclobutanone studied in this experiment was 2-dodecylcyclobutanone (2-DCB) which is formed from palmitic acid. Our objectives were to evaluate 2-DCB formation in fresh irradiated ground beef patties at two fat levels and to estimate the irradiation dose applied to commercial samples. Patties containing 15% and 25 % fat were irradiated by electron beam at 1.0, 2.0, 3.0 and 4.5 kGy. Commercially available one lb irradiated ground beef chubs with different fat levels (Brand X, 20% and 7% fat; Brand Y, 20% and 10% fat) were analyzed as well. The 2-DCB was extracted using supercritical fluid extraction (SFE) and analyzed by gas chromatography-mass spectroscopy (GC-MS). The GC-MS was operated in the selected ion monitoring mode, measuring ion m/z 98 and 112. The 2-DCB was detected in all the irradiated samples and its concentration increased linearly with dose (R2=0.9646 for 25% fat samples, R2=0.9444 for 15% fat samples). Moreover, there was no significant difference in 2-DCB concentrations between the two fat levels. The estimated doses applied to the commercial samples ranged between 1.38 kGy and 1.55 kGy, values consistent with doses normally used in the industry (1.0 - 2.0 kGy). Our results show that 2-DCB can be used to monitor fresh irradiated beef and approximate the dose applied to it. This is important in order to ascertain if retail ground beef has been irradiated within the legal dose limit.
Session 67C, Food Chemistry: Food analysis, irradiation and toxicology
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