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S. BANALA1, M. Brouk1, and K. A. Schmidt2. (1) Department of Animal Science and Industry, Kansas State University, 160 Call Hall, Manhattan, KS 66506-1600, (2) Food Science Institute, Kansas State University, Call Hall 224, Manhattan, KS 66506 In 2002, 38,874,226 kg of milk was disposed due to the presence of antibiotics, which can pose a serious health risk for selected populations. Antibiotics enter the general milk stream, when an antibiotic-treated cow is inadvertently milked with other healthy cows. Currently, technology does not exist to remove unwanted antibiotics from milk. b-lactam drugs which are one of the more popular class of drugs used to treat mastitis, have structures similar to other organic compounds that have been reported to form complexes with b-Cyclodextrin (b-CD). These complexes may be precipitated under certain conditions, thus, the test hypothesis was to determine if b-CD can complex and precipitate unwanted antibiotic residues in milk. The overall objective was to develop a methodology to complex b-CD with b-lactams for potential removal from raw milk. A varying ratio of solute (b-CD) to solvent (water or whole milk) was used to optimize b-CD crystallization conditions. Factors such as crystallization time (4-12 h), crystallization temperature (7 vs. 22 oC) and centrifugation speed (25 - 3000 x g) were studied. Optimized crystallization conditions were verified in antibiotic-tainted raw milk samples via the SNAP¢ç test. In water, b-CD precipitate increased significantly as b-CD concentration (2.5 - 15%) and crystallization time (5 - 10 h) increased, but was independent of the centrifugation speed. When whole milk was the solvent, similar trends were observed for b-CD concentration and crystallization time, but as centrifugation speed increased, precipitate solids increased. Eleven cows were treated with b-lactam antibiotic and 12 h later, milk was obtained and treated with b-CD. As b-CD concentration increased, larger precipitates were recovered and supernatants evaluated with SNAP¢ç test showed reduced levels of antibiotics. Results indicated that b-CD may have the potential to reduce the levels of b-lactam antibiotics in antibiotic-tainted raw milk.
Session 46, Dairy Foods: General
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