33F-23 |
|
Y. KIM1, I. U. Gruen2, R. S. MacDonald2, and L. N. Fernando2. (1) Food Resource Utilization Division, Korea Food Reseach Institute, San 46-1, baekhyun-dong bundang-ku, sungnam-si, kyounggi-do, 463-420, South Korea, (2) Dept. of Food Science, Univ. of Missouri- Columbia, 256 Eckles Hall - Stringer Wing, Columbia, MO 65211 Explosion of interest on herbal dietary supplements can be attributed to several social and economic factors, including consumer's natural product preference and overall dissatisfaction with the high cost and side effects of conventional medicine. Sutherlandia (common English name for Sutherlandia Frutescens subs. Microphylla) is a single stemmed plant with flowering and grows best in dry and stony grasslands of Southern Africa. This plant has been used hundreds years for remedy for stomach problems and internal cancers in African culture and also said to be a useful bitter tonic and a good general medicines. According to tradition, the virtues of the plant extend to include remedies for colds, influenza, diabetes, inflammation, liver problems, backache and rheumatism. Since no appropriate mechanism currently exists to evaluate the claimed therapeutic aspects of this herbal, the use of quantitative chemical methods is one way to evaluate herbal extracts on marker compound for its activity. To achieve this purpose, an HPLC assay focused on phenolic compounds was performed for samples prepared with techniques to concentrate active compounds. Saponins are often found, as numerous marker compounds for bio-functionality and those contents were also determined. The antioxidant activity and anti-glycation activity was observed in methanol extract and its partially purified samples with solvent fractionation and chromatographic techniques. The correlation coefficients between chemical markers and total antioxidative activity and anti-glycation activity of the methanol extract and partially purified samples were also determined. This research offers opportunities to concentrate bioactive compounds from sutherlandia and to evaluate their bioactivities, so that it's application in functional foods or medical food become more possible.
Session 33F, Nutraceuticals & Functional Foods: Antioxidants and phytochemical analysis
|