83B-6 |
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D. R. DIEGEL1, A. M. Nagy1, L. W. Fauque1, and C. L. Hicks2. (1) North Toole County High School, 414 3rd St. S., PO Box 170, Sunburst, MT 59482, (2) Dept. of Animal Sciences, Univ. of Kentucky, 410 W.P. Garrigus Bldg., Lexington, KY 40546-0215 To determine the effect of migrating compounds (plasticizers) from various plastics on Daphnia magna, selected plastics were heated in an oil medium. Vinyl, styrene, polyethylene, and polyethylene terephthalate were cut into small pieces (2 g), mixed with 10 ml of safflower oil and .05 ml of Tween 80, and heated (96°C) for 5, 10, and 20 min. After cooling, 2 mL of the heated oil was added to 200 mL of spring water. Five Daphnia magna and ca (.25 g) of Daphnia food were added to each beaker. Daphnia mortality rates, heart rates, reproduction, and activity were observed (once per day) for a period of seven days. Data were recorded and analyzed using T-tests and Orthogonal contrasts. The experiment was replicated 3 times. Daphnia placed in water containing heated oil without added plastic (control) had the greatest activity, heart rate and lowest mortality rates. Daphnia in media containing migratory compounds from vinyl, polyethylene, and PET had activity levels that were lower than the Daphnia in the control media but much higher than the Daphnia in the medium containing styrene migratory compounds. Heart rates decreased and mortality increased in media that contained migratory residues from plastics, however heart rates were highest and mortality was lowest in the medium containing polyethylene compounds whereas the styrene medium produced the lowest heart rates and the highest mortalities. Results indicate migratory compounds in vinyl, styrene, polyethylene, and polyethylene terephthalate were deleterious to Daphnia magna.
Session 83B, Food Packaging: Applications of edible coatings, antimicrobial films, and sensors in food packaging
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