114E-21 |
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S. O. SERNA SALDIVAR, Dept. of Food Technology, ITESM-Campus Monterrey, E.Garza Sada 2501 Sur, Monterrey, 64849, Mexico, J. A. Gutierrez-Uribe, Center of Biotechnology, ITESM-Campus Monterrey, Av. Eugenio Garza Sada 2501 Sur, Monterrey, 64849, Mexico, and R. H. Liu, Dept. of Food Science, Cornell University, Stocking Hall, Ithaca, NY 14853-7201. Plant phenolics, flavonoids and related bioactive compounds are a diverse group of phytochemicals that widely occur in a variety of plants and have gained attention due to their antioxidant capacity and other functions that benefits human health. Some of these compounds are effective phytochemicals against cancer cells. The objectives of this research were to quantify the amount and types of phenolics present in the seed coats of different black bean (Phaseolus vulgaris) varieties and test the anti-proliferative effects of the extracts on Caco-2 human colon cancer cells. Different varieties of beans were immersed in distilled water for 8 or more hours at room temperature. Then the seed coats were manually removed, collected, and dried. The seed coats were extracted using 80% acetone, and their total phenolic and flavonoid content was determined. Antiproliferative activities on Caco-2 human colon cancer cells were measured by the MTS assay. The weight of the seed coats averaged 7 to 13% (dry basis) in the varieties analyzed. Phenolics were concentrated mostly in the seed coats. Seed coats contained approximately 20 times more phenolics than whole grains. Total phenolics and flavonoids ranged from 84 to 277 and 20 to 48 mg catechin equivalents/gr (db), respectively. Anthocyanins and tannins ranged from 50-198 mg/L and 1.3 to 5 mg catechin/gr, respectively. All varieties had significant levels of anthocyanins delfidine, petunidine and malvidine. The proliferation of Caco-2 human colon cancer cells was significantly inhibited in a dose dependent fashion after exposure to the phytochemical extracts with EC50 values ranged from 119 to 469 µg/mL. Our results showed that phytochemical extracts from seed coats of black bean (Phaseolus vulgaris) have potential to be used as natural agents to prevent or treat colon cancer.
Session 114E, Nutraceuticals & Functional Foods: Bioactivity measurement and mechanism
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