114E-20 |
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S.-C. SHEU, Department of Food Science, National Pingtung University of Science and Technology, 1 HHseuh-Fu Rd., Neipu, Pingtung, 91201, Taiwan, H.-F. Lu, Institute of biotechnology, National Cheng-Kung University, No.1, Ta-Hsueh Road, Tainan, 701, Taiwan, H.-C. Chaung, Department of Verterinary Medicine, National Pingtung University of Science and Technology, 1, Hseuh-Fu Rd., Neipu, Pingtung, 91201, Taiwan, and Y.-Y. Lien, Departemnt of Veterinary Medicine, National Pingtung University of Science and Technology, 1, Hseuh-Fu Rd., Neipu, Pingtung, 91201, Taiwan. Because of environmental pollution and change of living style, the immune function of people is decreased. Therefore, it is important to find some materials from the nature to improve the immune function. Pines have been widely used for the medical purpose for a long time. Several studies reported that pine could regulate immune function. The objective was to determine the expression of interleukin-2 (IL-2), IL-4, IL-10, IL-18 and interferon-g (IFN-g) mRNA from mice splenocytes by reverse transcription-quantitative competitive polymerase chain reaction (RT- qcPCR) assay from in vivo and in vitro experiments to evaluate the immune function of Pinus morrisonicola Hay. In vivo, feeding with middle (19.5 mg/day/ mouse) and high (39 mg/day/mouse) concentration of pine needle extract could stimulate IL-2 and IFN-g expression. Without stimulation of ConA, only the expression of IL-2 and IFN-g increased after 4-week and 8-week feeding, respectively. At low concentration (1.95 mg/day/mouse) feeding, only the expression of IL-4 and IL-10 increased. The higher ratio of IFN-g/ IL-4 was found at the middle and high concentration feeding. The higher expression of IL-18 was found at the middle and high concentration feeding. In vitro, stimulation of middle (0.0975 mg/ml) and low (0.0195 mg/ml) concentration of pine needle extract could stimulate the expression of IL-2 and IL-4. Although the growth of splenocyte was inhibited by the high concentration (1.95 mg/ml) of pine needle extract but the expression of IFN-g still increased. These results indicated that different cytokines expression in Th1 and Th2 cells could be stimulated by different concentration of pine needle extract. Treatment with high and middle concentrations lead increase in Th1 cytokine levels, which suggested that cell-mediated immunity could be positively affected. But the low concentration treatment could up-regulate Th2 cytokine production toward humoral immunity.
Session 114E, Nutraceuticals & Functional Foods: Bioactivity measurement and mechanism
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