114E-7 |
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J. Y. AHN, T. Y. Ha, S. R. Kim, and C. K. Han. Functional Food Research Division, Korea Food Research Institute, San 46-1, Seongnam Si, 463-746, South Korea Butyl p-hydroxybenzoic acid (butyl paraben, BP) is one of a homologous series of parabens, and used as a preservative in food and cosmetic products. It is known that BP displays estrogenicity in both in vitro and in vivo analyses. The effect of ginseng treatment on the estrogenicity of BP was investigated. For that, we examined ginseng would exhibit oestorogenicity or not. Ginseng itself did not exert estrogenic effect in yeast estrogen receptor (YER) assay. Then, ginseng extracts were concurrently treated with BP. The ginseng extracts considerably inhibited activation of b-galactosidase by 100 mM BP and red ginseng than white ginseng, methanol extraction than water extraction were helpful to detoxify the effect of BP. For E-screen, ginseng extracts successfully suppressed BP-induced MCF-7 proliferation. The 6R inhibited MCF-7 proliferation induced by 10 mM of BP dose-dependently in both water and 70% methanol extract condition. In uterotrophic assay, ginseng attenuated uterine hypertrophy induced by BP. And for Hershberger assay, ginseng exerted anti-androgenic activity and inhibited testosterone-induced hypertrophy of reproductive system. Results reported in this study suggested that ginseng may be effective to reduce toxicity of BP in vitro and in vivo.
Session 114E, Nutraceuticals & Functional Foods: Bioactivity measurement and mechanism
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