114E-19 |
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J.-M. YUN1, S.-H. Lee2, H. Kwon3, and J.-K. Hwang2. (1) Bioproduct Research Center, Yonsei University, 134 Sinchon-dong, Seodaemun-gu, Seoul, 120-749, South Korea, (2) Department of Biotechnology, Yonsei University, 134 Shinchon-dong Sudaemun-gu, Seoul, 120-749, South Korea, (3) Department of Food & Nutrition, Seoul National University, San 56-1, Shillim-dong Kwanak-gu, Seoul, 151-742, South Korea Panduratin A is a phenolic compound isolated from Kaempferia pandurata (Zingiberacease family), which has been used as an edible plant for salads and spices in some tropical countries.K. pandurata has been known to possess anti-inflammatory and anti-carcinogenic effects. We previously reported that panduratin A was a cyclooxygenase-2 (COX-2) inhibitor in RAW 264.7 cells, indicating its effect as an anti-inflammatory nutraceutical. Many studies showed an increase of COX-2 expression due to inflammation in numerous cell lines, especially in colorectal cancer cells. It is thus conceivable that panduratin A may be related to the modulation of colon cancer cell. In the present study, we investigated the effect of panduratin A on the proliferation and apoptosis in the human colon cancer cell lines. An active compound was isolated from the methanol extract of K. pandurata by silica gel column chromatography, and its structure was confirmed as panduratin A by 1H-NMR, 13C-NMR, and FAB-MS. Cell proliferation and induction of apoptosis was determined by the MTT assay, DNA fragmentation measurement, flow cytometric analysis, and Western blot. On MTT assay, panduratin A markedly showed inhibitory effects on HT-29 (IC50: 25 mM) and COLO 201 (IC50: 7.8 mM) cell proliferation. The panduratin A-treated cells exhibited distinct morphological features of apoptosis such as chromatin condensation and internucleosomal DNA fragmentation. Flow cytometic analysis of HT-29 and COLO 201 treated with panduratin A showed the appearance of a sub-G1 peak representing the subdiploid cell population. Furthermore, treatment with an apoptosis-inducing concentration of panduratin A induced proteolytic cleavage of poly (ADP-ribose) polymerase (PARP) and pro-caspase 3 protein in HT-29 and COLO 201 cells. The results of this study strongly suggested that panduratin A, inducing growth inhibition and apoptosis in human colon cancer cells, would be potentially employed as an anti-carcinogenic nutraceutical.
Session 114E, Nutraceuticals & Functional Foods: Bioactivity measurement and mechanism
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