99A-28 |
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B. KIM1, X.-L. Su2, and Y. Li2. (1) Department of Food Science, University of Arkansas, Center of Excellence for Poultry Science, O-419 Poultry Science Building, Fayetteville, AR 72701, (2) Department of Biological and Agricultural Engineering, University of Arkansas, 230 Engineering Hall, Fayetteville, AR 72701 The incidence of foodborne diseases caused by Salmonella Typhimurium still remains as a problem in the United States. It is believed that poultry and poultry products can be vehicles of foodborne salmonellosis because the raw product may be initially contaminated with S. Typhimurium cells and may be transmitted to humans due to subsequent undercooking, cross-contamination, or improper thawing. Conventional microbiological isolations and identification methods are labor intensive and time consuming. There is a need for more rapid, sensitive and specific methods that will facilitate identification of contaminated foods. The objectives of this research were to develop a capillary immunosenosr for rapid detection of S. Typhimurium, and to evaluate its application to the analysis of poultry products. Samples containing S. Typhimurium were pumped into the fused-silica capillary column which had antibodies immobilized to capture the target bacteria in the samples and the enzyme labeled antibodies passed through the column. The number of the cells was determined by measuring the amount of the phenol produced by the enzyme reaction with phenyl phosphate substrate using a bienzyme electrochemical detector. Chicken carcass wash water and ground turkey meat were tested. The detection limit for chicken carcass wash water samples was 104 CFU/ml. The results showed a linear relationship between peak current and the cell number of S. Typhimurium in the range of 104 - 106 CFU/ml, with R2=0.9878. This detection can be finished within 2.5 h, which is faster than ELISA, PCR and conventional plating methods. However, to meet the requirement of the poultry processing industry, the detection limit still needs to be lowered further. Nevertheless, this method has a potential for rapid detection of S. Typhimurium in poultry samples.
Session 99A, Biotechnology: General
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