114E-18 |
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Y. O. PARK1, L. G. Yoo1, E.-S. Hwang2, and T. W. Moon1. (1) Department of Food Science and Technology, Seoul National University, School of Agricultural Biotechnology, San 56-1, Shillim-dong, Kwanak-gu, Seoul, South Korea, (2) Center for Agricultural Biomaterials, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul, South Korea Lycopene is the predominant carotenoid in tomatoes and tomato-based foods. There are many animal and epidemiological studies that tomatoes or lycopene can protect our body from various cancers, especially prostate cancer. It has been suggested that reactive oxygen species play an important role in the development of cancer. Lycopene is the most powerful singlet oxygen quencher and free radical scavenger. There are few studies on liver cancer and lycopene. Liver cancer is the second most common fatal cancer in Southeast Asia and Korea. The objectives of this research were to investigate the effect of lycopene on DNA damage as well as cell growth inhibition in Hep3B human hepatoma cell line. Lycopene is highly hydrophobic which is problematic in aqueous cell culture medium. Therefore, 10% water solubilized lycopene (Roche Inc.) was used with placebo (5% triacylglycerol). Lycopene was analyzed by HPLC and cell proliferation was determined by MTT assay. The cells were plated in 96-well plates and a final lycopene concentration of 0.1 - 50 uM was added. After 24 h, cell viability measured with an ELISA reader at 570 nm. Cellular oxidative DNA damage was determined by Comet assay (single cell gel electrophoresis). Lycopene inhibited cell growth in a dose dependent manner and growth inhibition was 20% and 40% at 0.2 uM and 50 uM lycopene, respectively after 24 h incubation. In Comet assay, lycopene treated cells showed less DNA damage than those without treatment. Our study demonstrated the antitumor properties of lycopene on Hep3B cell growth. Lycopene, therefore, deserves study as a potential chemopreventive agent.
Session 114E, Nutraceuticals & Functional Foods: Bioactivity measurement and mechanism
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