114E-17 |
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H.-C. CHIANG and G.-C. Yen. Dept. of Food Science, National Chung Hsing Univ., 250 KuoKuang Road, Taichung, 40227, Taiwan Intestinal damage and bleeding are the major side effects of non-steroidal anti-inflammatory drugs (NSAIDs). Researches have been indicated that oxygen radical-dependant microvascular injury may be an important event of all on intestinal mucosal damage. Diets rich in antioxidants play an important role in protecting the intestinal tract itself from oxidative damage, and therefore may protect mucosal cell form NSAIDs-induced ulceration. The present study evaluates the protective effect of 11 antioxidants on NSAIDs-induced oxidative damage in Int 407 cells. This study focused on screening the protective effect of 11 antioxidants, including catechin, epigallocatechin gallate (EGCG), theaflavin, caffeic acid, protocatechuic acid (PCA), furulic acid, lycopene, beta-carotene, cyaniding, malvidin, and apiginidin, on ketoprofen-induced oxidative damage in Int 407 cells. Int 407 cells were pre-incubated with 11 antioxidants in a proper time period (based on experimental data), and then treated with 200 uM ketoprofen for 1 h (antioxidants were removed before ketoprofen treatment). The contents of malondialdehyde (MDA), total thiol groups (TSH), 8-hydroxy-2-deoxyguanosine (8-OHdG) as well as the activities of intracellular glutathione peroxidase (GPx) and glutathione reductase (GRd) were determined to evaluate the protective effect. Catechin, EGCG, caffeic acid, and PCA significantly (p<0.05) reduced the MDA and 8-OHdG content; however, they increased the intracellular GPx activity to a normal level. Beta-carotene also reduced the MDA content but it had influence on GPx activity. All of the 11 antioxidants did not alter the GRd activity and TSH content when compared with ketoprofen treatment only. Anthocyanins that tested in this experiment (cyanidin, malvidin, and apiginidin) seemed have no effects on ketoprofen-induced cell damage. Among the 11 antioxidants, catechin, EGCG, caffeic acid, and PCA may have potential benefit on preventing intestinal ulcerogenic injuries.
Session 114E, Nutraceuticals & Functional Foods: Bioactivity measurement and mechanism
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