33G-9 |
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C. E. MARTÍNEZ-SÁNCHEZ1, E. Herman-Lara1, H. S. Garcia2, V. Santos1, C. Lopez1, and M. Villarreal1. (1) Biochemical Engineering Dept., Instituto Tecnologico de Tuxtepec, Calz. Dr Victor Bravo Ahuja s/n, Col. 5 de Mayo, Tuxtepec, Oax., 68360, Mexico, (2) UNIDA, Instituto Tecnologico de Veracruz, M.A. de Quevedo # 2779, Col. Formando Hogar, Veracruz, Ver., 91897, Mexico At present there is great interest in conjugated isomers of linoleic acid (CLA), because they have been shown in many studies to have various protective physiological properties. The most commonly described effect of CLA is the reduction of carcinogenesis. In a previous work CLA intake was estimated to be several hundred milligrams per day. This level of intake is considerably less than the 3 g/day minimum value extrapolated from animal studies that would result in beneficial effects. However, exact intake levels in humans that confer these effects are still unknown. Nevertheless, increasing CLA consumption or assessment of its consumption requires documentation of CLA composition and content in the food supply. The purpose of this study was to quantify the CLA content in some commonly consumed Mexican foods and to estimate an average CLA daily intake. Samples were obtained locally and analyzed as both raw and cooked preparations from 20-50 g. Lipid material was extracted in duplicate according to the method of Folch et al. (1957). Fat content was determined from dry lipid weight. After methylation, lipid extracts were analyzed by GC. Under the present experimental conditions only the cis-9, trans-11 isomer was detected. Other minor isomers, which may be present, were not determined by the method used in this study. Levels of CLA ranged between 0.83 to 9.335 mg/g of fat. On the basis of a 100 g serving size of the foods studied, levels of CLA ranged between 74.0 and 114.28 mg per portion. This study showed the presence of CLA content in dairy and meat products and daily intake levels could be increased consuming different types of foods.
Session 33G, Nutraceuticals & Functional Foods: Lipid and probiotic functional foods
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