79-10 |
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E.-J. KIM, Food Function Research Divison, Korea Food Research Institute, San 46-1, Baekhyundong, Bundanggu, Songnamsi, Kyunggido, 463-746, South Korea, S. Park, Food Function Research Division, Korea Food Research Institute, San 46-1, Baekhyundong, Bundanggu, Sonnamsi, Kyunggido, 463-746, South Korea, and S. H. Yoon, Food Function Research Division, Korea Food Research Instittue, San 46-1, Baekhyundong, Boondanggu, Songnamsi, Kyunggido, 463-746, South Korea. Relatively little research deals with structuring lipids by enzymatic reactions in solvent-free systems that advantageously requires fewer and easier purification steps than solvent systems. The objective of the present study was to produce 1,3-diacylglycerol using lipases by esterification of capric acid and glycerol in a solvent-free system. Reactions were conducted according to 3 X 2 X 3 factorial arrangements composed of temperature (40, 50, or 60oC), enzyme amount (5 or 10%), and substrate ratio (1:1, 1:2, or 1:3). Production of monocaprin, dicaprin, and tricaprin were quantified using a reversed-phase HPLC system. Yield of mono- and dicaprin reached maximum after 4hr reaction and decreased thereafter (p<0.0001), whereas yield of tricaprin (p<0.05) and total glyceride (p<0.0001) steadily increased up to 24hr reaction. Reaction temperature affected the production of mono- (p<0.0001), di- (p<0.0001), and tricaprin (p<0.05). Higher reaction temperature (50 or 60oC) resulted in more glycerides production. Production yield of mono- (p<0.001) and total glyceride (p<0.0001) was affected by amount of lipase added. The more lipase (10% (w/w)) was shown to induce more rapid esterification. Concentration of substrates affected yields of dicaprin (p<0.01) and total glyceride (p<0.01) production. The higher ratio of glycerol to capric acid (1:2 or 1:3) resulted in the more glyceride production with the yield of 63 and 66%, respectively. The results of the study indicated that the reaction at 60oC temperature with 10% enzyme and 1:3 substrate ratio would be optimal among conditions tested. Structuring lipids from capric acid and glycerol were easily performed in a solvent-free system which is applicable to develop a continuous bed-reactor.
Session 79, Food Chemistry: Protein and enzyme chemistry
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