99A-11 |
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G. GONZÁLEZ-RUIZ, E. Mercado-Silva, and R. Martínez-Peniche. Departamento de Investigación y Posgrado en Alimentos, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, Querétaro, 76010, Mexico Mexico imports large amounts of garlic from Chile, but signs of garlic illegally introduced from China passing through Chile have been detected; it is sold at prices that are lower than those paid for the garlic locally produced. This abnormal situation affects the national production causing a reduction in the area destined to this crop. In order to demonstrate this practice of dumping, it could be useful to find the molecular banding patterns of garlic related to the genetic origin. DNA markers have proven to be extremely efficient to discriminate and identify different individuals. RAPD (Randomly Amplified Polymorphic DNA) markers detect polymorphism in different individuals due to mutations or changes in DNA sequence. The objective of this study was to recognize specific DNA banding patterns of garlic cultivars from different origins using RAPD markers. DNA was extracted from fresh young leafs of four garlic samples from China, Chile, Taiwan and Singapore and analyzed using 36 RAPD primers. Amplified PCR fragments were visualized under UV light following separation on an agarose gel and stained with ethidium bromide. Total and polymorphic bands were recorded. Only eight primers produced significant PCR banding patterns and showed polymorphism. Seventy DNA fragments were amplified from these primers; 42 (60 %) of them were polymorphic and 28 (40 %) were monomorphic. Four RAPD specific markers were found in the Chinese sample (OpB16=1300 pb; OpB16 > 2072 pb; OpC05=2072 pb; OpN17 > 2072 pb) and one in the Taiwanese sample (OpO18=1300 pb). Five RAPD markers related to Chinese and Taiwan cultivars were found. Genetic markers could be used as a tool to identify the origin of garlic cultivars.
Session 99A, Biotechnology: General
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