99E-6 |
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A. MORA-GUTIERREZ1, V. J. McWhinney1, and C. Wang2. (1) Cooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX 77446, (2) Human Nutrition Research, Kentucky State University, Frankfort, KY 40601 Phosphorylated peptides originating from milk proteins containing unique anionic clusters are known as caseinophosphopeptides (CPP). These peptides form soluble complexes with calcium in the digestive tract thereby enhancing calcium absorption. Bovine and caprine CPP differ in the casein ratio (as1- to as2-casein in particular) which may display specific effects on bone mineralization; therefore, this research evaluated these compositional characteristics in male rats. Three-week old Sprague-Dawley male rats were divided into groups of ten for baseline data, control and test diets. The control and test diets contained 0.5% Ca and 0.4% P. The test diets (i.e., bovine and caprine CPP) provided 100% of dietary Ca and 100% of dietary P. The rats were fed with the diets and double deionized water for 56 days ad libitum, their feed intake was noted daily, and their weights were recorded once a week. Thereafter, the rats were killed, and their bones (femurs) were excised. The right femoral bones were analyzed for minerals (Ca, Mg and P) by atomic absorption spectrophotometry. The left femoral bones were scanned on a bone densitometer to determine the bone mineral content (BMC) and bone mineral density (BMD). Femoral Mg and P concentrations were unaffected by dietary bovine or caprine CPP. While femoral Ca concentration (mmol/g) was increased by dietary bovine or caprine CPP (control: 5.08; bovine CPP: 5.36; caprine CPP high as1: 5.38; caprine CPP low as1: 5.39). Bovine and caprine CPP were associated with greater femoral BMC (g/cm) (control: 0.1921; bovine CPP: 0.2049; caprine CPP high as1: 0.2044; caprine CPP low as1: 0.2050) and BMD (g/cm2) (control: 0.1264; bovine CPP: 0.1352; caprine CPP high as1: 0.1351; caprine CPP low as1: 0.1354). These results suggest that bovine or caprine CPP could be administered in the treatment of bone disease due to lack of normal calcium utilization.
Session 99E, Nutrition: General II
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