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M. C. QIAN1, K. Klesk1, and R. R. Martin2. (1) Dept. of Food Science & Technology, Oregon State Univ., 244-A Wiegand Hall, Corvallis, OR 97331-6602, (2) Northwest Center for Small Fruits Research, USDA-ARS-Horticultural Crops Research Laboratory, 3420 N.W. Orchard Ave., Corvallis, OR 97330-5098 Raspberries are rich sources of anthocyanins and phenolics. Red raspberries are seeing increased use by consumers as an important part of a healthy diet. Washington and Oregon are the major red raspberry producing states. ‘Meeker’ red raspberry is the major cultivar grown in this region due to its high yield and superior fruit quality. However, ‘Meeker’ red raspberry is susceptible to raspberry bushy dwarf virus (RBDV) which causes crumble fruits. Efforts have been made to develop RBDV resistant ‘Meeker’ red raspberry through transgenic engineering. This study is focused on comparison of volatile composition of transgenic red raspberries with wild type ‘Meeker’ red raspberry. Fruits from five transgenic plants were collected in the summer of 2003. In addition, wild type ‘Meeker’ fruits were collected from both Oregon and Washington states. Solvent extracted red raspberry volatiles were analyzed with gas chromatography-mass-spectrometry (GC-MS) technique. Seventy-six compounds were identified by mass spectral data, and were common to all seven cultivars. Quantification of the compounds was made by comparing each compound’s peak area to the peak area of an internal standard (ethyl undecanoate). Generally, there is little variation in compound concentrations between the cultivars studied. However, several trends were noticed. The Washington ‘Meeker’ appears to have atypical amounts of hexanal, t-3-hexenoic acid, and d-decalactone compared to the other samples. The wild Oregon fruit appears to have less vanillin, while transgenic 2174BS9-7 has a large amount of d-decalactone. Transgenic 2172BJ has a large amount of a-pinene, but no detectible amount of the raspberry ketone (4(p-hydroxyphenyl)-2-butanone). Finally, transgenic 2174BH5-6 appears to have larger amounts of hexanal and acetic acid than the other samples. This research provides information on the type of variability in fruit quality parameters in the transgenic red raspberry plants. Since we have multiple lines with good resistance to RBDV, we can pick and choose among these lines to target a plant with the best fruit quality.
Session 110, Biotechnology: General
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