33F-11 |
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D. V. BANGALORE, D. D. Scott, and W. G. McGlynn. Oklahoma Food & Agricultural Products Research & Technology Ctr., Oklahoma State Univ., 148 FAPC, Stillwater, OK 74078-6055 Lycopene,a lipophyllic antioxidant, has a crucial role in biological systems as an anti-cancer agent, lowering cardiovascular risks and in regulating the immune system. Oxygen radical absorbance capacity (ORAC) as an index for antioxidant activity has been validated extensively for hydrophilic antioxidants but its applicability to lycopene has not yet been fully demonstrated. This study validates the ORAC assay for different concentrations of lycopene in presence of b-cyclodextrin, a water solubility enhancer. Lyc-O-MatoTM 6 % extract was used as a source of lycopene for our experiments. Lycopene was extracted by standard spectrophotometric assay procedure in the presence of b-cyclodextrin at concentrations of 0, 0.4, 0.8 & 1.6 % and the antioxidant activity of lycopene at different levels measured with the ORAC assay. Experiments were conducted in quadruplicates and statistical pooled correlations analyzed. Statistical analysis showed a very high correlation (R2=0.99) between ORAC and ascorbic acid concentrations. Lycopene concentration correlated poorly with ORAC (R2=0.27) in absence of b-cyclodextrin. Correlations improved with increasing levels of b-cyclodextrin (R2=0.51 and 0.9 for 0.4 and 0.8 % b-cyclodextrin respectively). A very high b-cyclodextrin concentration (1.6%) decreased the correlation between ORAC and lycopene concentration. Inclusion of b-cyclodextrin in the ORAC assay improves correlation between ORAC and lycopene concentration, thus expanding the scope of the ORAC assay to include fat soluble antioxidants.
Session 33F, Nutraceuticals & Functional Foods: Antioxidants and phytochemical analysis
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