83B-14 |
|
Y. SONG1, R. Gerdes2, M. D. Nicholson3, and M. L. Tortorello1. (1) National Center for Food Safety & Technology, U.S. Food & Drug Administration, IIT Moffett Campus, 6502 S. Archer Rd., Summit-Argo, IL 60501, (2) National Center for Food Safety & Technology, Illinois Institute of Technology, IIT Moffett Campus, 6502 S. Archer Rd., Summit-Argo, IL 60501, (3) Research & Technology, Viskase Corp., 625 Willowbrook Centre Pkwy., Willowbrook, IL 60527 Recent Listeria outbreaks in deli meat products have expanded industrial interest in antimicrobial packaging of these products using nisin. Peel fibrous film is commonly used in processing of deli meats in industry. However, there is little published information pertaining to the applicability of nisin on peel fibrous film and its bactericidal effect on deli meat products. This research was conducted to evaluate the effectiveness of nisin-coated peel fibrous film in inhibiting the growth of Listeria monocytogenes on bologna meat. Nisin-treated peel fibrous film with a surface concentration of 1.2 mg nisin per 100-in2 film was produced using a coating procedure. Commercial bologna production and storage conditions were simulated as follows. A piece of bologna (3.5 x 4.0 x 0.0625 in) was sandwiched between nisin-treated films, processed at 162oF for 20 minutes, inoculated with a streptomycin (Sr) resistant strain of Listeria monocytogenes at 104 CFU/slice inside a plastic bag. The package was vacuumized, heat-sealed, and then stored at 4 and 10oC for 4 weeks. Samples were taken every 7 days, and the populations of surviving Listeria monocytogenesSr were recovered from the samples using a stomaching procedure and enumerated on tryptic soy-yeast extract agar supplemented with .01% streptomycin. Studies showed a 2 log reduction in Listeria monocytogenesSr populations immediately upon contact with the nisin-treated film, even at the treatment level 5 times less than the current regulatory limit. After 28 days of refrigerated storage, Listeria monocytogenesSr populations from nisin-treated samples were significantly less than (P<0.05) control samples; log10 1 vs 4 CFU/g for 4oC storage and log10 6 vs 8 CFU/g for 10oC storage, respectively. These results suggest that nisin-coated fibrous film may be applicable to inhibit growth of Listeria monocytogenes in deli meat products such as bologna.
Session 83B, Food Packaging: Applications of edible coatings, antimicrobial films, and sensors in food packaging
|