49I-2 |
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M. D. C. VALADEZ VEGA1, G. Alvarez Manilla Dubón, A. García Carrancá, and F. Abdullaev. (1) Biochemistry, Universidad Autónoma del Estado de Hidalgo, Carr. Pachuca-Tulancingo Km. 4.6, Pachuca, 42076, Mexico Lectins are sugar-binding proteins of non-immune origin which agglutinate cells and precipitate glycoconjugates. Due to their specific properties, lectins are used as a tool both for analytical and preparative purposes in biochemistry, cellular biology and immunology, as well as a diagnostic for therapeutic purposes in cancer research. Our target was to study the cytotoxic effect of tepary bean lectins on four human malignant cells lines. We purified lectins by affinity chromatography, using minileak-fetuin column. The cytotoxicity assay was carried out using four human malignan cells lines, Sknsh, Sw480, MCF-7 and C33-A. The cells were cultured in microplates and the cytotoxic effect and ID50 were determinated culturing the cells lines 24 h with lectins (0.0 to 400 ug/mL) and then, they were analyzed by MTT assay. Proliferation assays were carried out culturing the cells with lectin (0.0 to 100 ug/mL) in microplates for 24 h, and the cell proliferation was analysed by MTT and (3H) thymidine assay. The results showed that MCF-7 cells were more sensitive to cytotoxic effect (ID50 61.8 ug/mL) while C33-A cells were more resistant to lectin effect (major 400 ug/mL). The effect on the cell proliferation by MTT assay showed that the SW480 cells were more affected, while Sknsh, MCF-7 and C33-A were lightly affected. On the other hand, the (3H) thymidine assays showed that the lectins are more cytotoxic on MCF-7 cells, followed by SW480, while Sknsh cells were not affected. This work demonstrates that plants that are wild or semi-domesticated, which contain anti-nutritional products such as lectins, can be used to obtain added value products with therapeutic or diagnostic applications that are alternative to their use as food.
Session 49I, Toxicology & Safety Evaluation: General
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