114E-13 |
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H.-K. KIM1, M.-G. Choi1, C. Choi2, J.-H. Kwon3, and Y. Jeong4. (1) Dept. of Food Chemistry & Biotechnology, Korea Food Research Institute, San 46-1, Baekhyun-Dong, Bundang-Ku, Songnam, Kyonggi-Do, 463-420, South Korea, (2) Dept. of Food Science & Technology, Yeungnam Univ., 214-1 Dae-Dong, Kyungsan, Kyungbook, 712-749, South Korea, (3) Dept. of Food Science & Technology, Kyungpook National Univ., 1370 Sankyuk-Dong, Buk-Gu, Daegu, 702-701, South Korea, (4) Dept. of Food Science & Nutrition, Dankook Univ., San 8 Hannam-Dong, Yongsan-Ku, Seoul, 140-714, South Korea Vegetables such as broccoli, kale and cabbage contain phytochemicals as well as vitamins and minerals. Phytochemicals in cabbage include caffeic acid, chlorogenic acid and ferulic acid, r-coumaric acid which are reported as antioxidants. The objective of this study was to characterize physiological activities such as electron donating ability, SOD-like activity, nitrite scavenging activity and the tyrosinase inhibitory activity of Brassica oleracea var. capita extracts. Three varieties of Brassica oleracea var. capita (Bonus, Summer power and Ruby star) were cleaned, air-dried at 45C°Æ, ground and extracted using water and ethanol (50 and 100%) for one hour. Electron donating ability was measured using a, a-diphenyl-picrylhydrazyl. SOD-like activity was assayed using pyrogallol as substrate. The nitrite scavenging activity of Brassica oleracea var. capita extracts was tested at pH 1.2, 3.0. 4.2 and 6.0. Commercial mushroom tyrosinase was used to measure inhibition by Brassica oleracea var. capita extracts. Extraction yields were in the order of water>50% ethanol>100% ethanol. Total contents of polyphenol were highest in the water extracts of Ruby star (424.50°¾10mg%). Electron donating abilities of Ruby star extracts were higher (86.70-99.54%) than those of Bonus and Summer power extracts. The water extract of Ruby star showed 10.44% of SOD-like activities while the other two water extract showed no SOD-like activity. In all ethanol extracts SOD-like activities were from 41.62% through 67.44%. The tyrosinase inhibitory activities of all samples were highest in the 50% ethanol extracts. Nitrite scavenging activities at pH 1.2 were the most effective in water and 50% ethanol extracts of all the samples. The results of this study will be useful for understanding the physiological activities of Brassica oleracea var. capita extracts.
Session 114E, Nutraceuticals & Functional Foods: Bioactivity measurement and mechanism
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