99A-2 |
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J. L. NAVARRETE_BOLAŅOS1, O. Serrato-Joya, H. Cardenas-Arzate, C. Luna-Guiza, E. Botello-Alvarez, H. Jimenez-Islas, and R. Rico-Martinez. (1) Departamento de Ingenieria Quimica-Bioquimica, Instituto Tecnologico de Celaya, Av. Tecnologico s/n, Celaya, Guanajuato, 38030, Mexico Provitamin A activityis present in a limited number of carotenoids. Carotenoids which possess one un-substituted beta end group are converted, in mammals, to vitamin A. The principal provitamin A carotenoids occurring in the nature are a-carotene, b-carotene, and b-cryptoxanthin. Since alpha-carotene and b-cryptoxanthin, each have only one un-substituted font beta end group, the provitamin A activity is calculated to be only one-half of that of the b -carotene. Because of the relatively large amounts of b-cryptoxanthin in many fruits and vegetables, this carotenoid can be considered as the main source of provitamin A. However, most of the information on provitamin A activity is based on the amount of b-carotene alone. The reason b-cryptoxanthin has not been given more importance is the lack of suitable analytical techniques, and alternative viable processes for production other than extraction from natural sources. Recent studies have indicated the potential health benefits of a diet rich in carotenoids. Apart from their provitamin A capacity, several studies showed carotenoids to be important for several other benefits. For example, as antioxidants, as preventing agents against cardiovascular diseases, age-related macular degeneration, and cataracts. Owing to the great interest in the role of carotenoids in human health, in this study we develop a novel alternative method to obtain b-cryptoxanthin. Flavobacterium sp obtained from ATCC (21588) was used in the fermentation assays. As optimization strategy, we used a experimental sequence, based on experimental design. The experimental results analysis showed that the maximum synthesis of b-cryptoxanthin (800 ppm) is reached when the fermentation process, in a stirred vessel, takes place at 27.5 °C, 0.09 volume of air per volume of medium, 195 rpm of agitation and pH of 7.9. HPLC analysis shows that the 95% of product is b-criptoxanthin. The reached concentration surpass the values reported for natural sources and others similar processes to obtain carotenoids.
Session 99A, Biotechnology: General
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