99A-1 |
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J. L. NAVARRETE_BOLAŅOS1, C. L. Rangel-Cruz, J. Zavala-Nigoa, K. S. Peņuņuri-Lara, E. Botello-Alvarez, H. Jimenez-Islas, and R. Rico-Martinez. (1) Departamento de Ingenieria Quimica-Bioquimica, Instituto Tecnologico de Celaya, Av. Tecnologico s/n, Celaya, Guanajuato, 38030, Mexico Marigold flower pigments (xanthophylls) are used as additive to poultry feed due to its pigmenting properties, and as human nutritional supplement based on important biological functions as nutraceuticals. Several alternatives have been developed for xanthophylls production, however, none of them reports production in larger quantities than those obtained from natural source. The commercial xanthophylls extraction from marigold involves: ensilage, pressing, drying, and extraction. In previous work, we studied marigold ensilage seeking to improve xanthophylls extraction and demonstrated that the saprophytes microorganisms Flavobacterium IIb, Acinetobacter anitratus and Rhizopus nigricans, are the most significant in ensilage and exhibit high cellulase synthesis. These enzymes are associated to structural wall cells petals degradation and their function is improving xanthophylls extraction. Previously, it was shown that enzymatic treatments, based on commercial cellulases, can advantageously improve xanthophylls extraction. However, these methods had practical limitations, namely long reaction times and high commercial enzymes cost. In this contribution, we analyze the enzymatic treatment effect using raw enzymatic extract from saprophytes microorganisms, and commercial cellullase, to obtain marigold flours with high xanthophylls contents and increase extraction yield using hexane. The products obtained from each treatment were analyzed to determine the total xanthophylls concentration and yield extraction by hexane. The results show that xanthophylls extraction from marigold flower can be improved using a raw enzymatic extract. Clearly, the process described has significant advantages when compared to traditional commercial processes and similar previous studies that use a commercial cellulase: 1) the treatment with hydrolytic enzyme synthesized by microorganisms associated as normal flora of marigold flower is more cost-efficient due in part to the straightforward enzyme synthesis, 2) a substantial reduction in processing time can be achieved while attaining high xanthophylls content, and 3) this enzymatic treatment exhibit more favorable mass transfer rates that allow us to recover nearly 95% of xanthophylls.
Session 99A, Biotechnology: General
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