49I-22 |
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E. Massarollo, Animal reference laboratory, Ministry of agriculture, Estrada da Ponta grossa 3036, Porto Alegre, 91780-580, Brazil and E. S. SANT´ANNA, Food Science and Technology Department, Santa Catarina Federal University, Av. Admar Gonzaga 1346, Florianópolis, 88034-001, Brazil. Avermectins are pesticides widely used in agriculture and it represents a risk for honey consumers due to the possibility of contamination through of the bee polinization. Considering the use of the avermectins as pesticides, there is the need to development of methods for detection of these compounds in honey. The aim of this study was the development of a new and rapid method for determination of avermectin B1a in honey. The method was carried out in honey samples fortified with reference solutions at three concentration levels - 0.016 mg/Kg; 0.023 mg/Kg and 0.039 mg/Kg. The analite was extracted from the matrix through liquid-liquid extraction with methanol/ethilacetate/hexane, followed by evaporation to dryness and clean-up in Solid Phase Extraction cartridges packed with Silica. Separation was run isocratically by High Performance Liquid Chromatography using a Reverse Phase column and mobile phase consisting of Acetonitrile:Methanol:Water. Detection was carried out by UV detector at 245 nm, so that the equipment response to the analite was linear, yielding correlation coefficients as great as 0.999. The results showed average recoveries between 73.4% and 97.45%, and Relative Standard Deviations ranging from 0.91% to 13.7%. In addition, under these stablished conditions it might be possible to quantify the (Z)-8,9 isomer formed through photodegradation of avermectin B1a. The method was rapid and accurate, so it could be employed in routine analyses.
Session 49I, Toxicology & Safety Evaluation: General
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