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G. J. LEYER1, V. Denin2, D. Brassart1, and B. Pot2. (1) Rhodia Inc., 2802 Walton Commons W., Madison, WI 53718, (2) Institut Pasteur de Lille, Lille, France Probiotic lactic acid bacteria consumption has been documented to reduce the incidence and duration of specific diarreal diseases and their role in immune system modulation has been cited as increasing in vitro pathogenic phagocytosis, controlling allergic inflammation at early ages, and acting as an immune system adjuvant. In order to better select strain(s) for targeted immunomodulatory health benefits and potential inclusion into human clinical trials, an in vitro and in vivo screening approach was implemented. A variety of probiotic cultures were studied for immunomodulatory parameters in an attempt to select strains with an elevated anti-inflammatory potential. Strains naturally present in foods and from a commercial strain collection have been screened for their immunomodulation properties by measuring cytokine expression profiles of human peripheral blood mononuclear cells (PBMC). The diverse immunomodulation profile was strain-specific. A limited number of strains displayed a predominant anti-inflammatory profile while a majority of strains showed a more neutral (moderate induction of all cytokines) or a pro-inflammatory profile. The in vitro test results were validated by in vivo animal trials using a TNBS-induced colitis model in mice. Selected strains with an anti-inflammatory immunomodulation profile were very effective in reducing the intestinal damage (colitis) in mice after intra-rectal administration of TNBS. The strains with a pro-inflammatory profile were not able to reduce the colitis symptoms. The most performing strains have been selected for inclusion in an ongoing human clinical trial. In general, the results of immunomodulation tests on PBMC and the performance of the strains in an in vivo animal model of colitis correlated well. The applicability of this mouse model to select the most performing anti-inflammatory strains for applications in human disease situation is currently being evaluated.
Session 24, Probiotics in health: Their potential against allergic diseases
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