45I-22 |
Random centroid optimization of lutein enriched salad dressing emulsions |
A. KHACHATRYAN, M. Ogawa, and J. N. Losso. Dept. of Food Science, Louisiana State Univ. Agricultural Center, 111 Food Science Bldg., Baton Rouge, LA 70803-4200 Age-related macular degeneration (AMD) is a physiological condition that affects old people, diabetic patients, and leads to loss of independence. Effective therapies vs AMD are non existent. The role of nutrition in preventing AMD is receiving strong scientific scrutiny. Lutein and zeaxanthin have been identified as dietary strategies that can delay the onset of AMD. Consumption of at least six milligrams of lutein per day has been reported to delay the onset of AMD by as much as 43%. However, most available food sources contain little amount of lutein to meet the daily requirement. It would require 2 lbs of corn, 16 lbs of tomatoes, one salad bowl of kale to meet the minimum daily requirement. As a result food fortification with lutein extract is appealing to health conscious consumers. The objective of this study was to prepare a salad dressing emulsion containing at least 6 mg of lutein per serving size (one tablespoon) as a daily source of lutein. Random centroid optimization was applied to find the best conditions for preparing a stable emulsion. Experimental parameters included: lutein concentration (0.010-0.020%); pH (3.5-4.5); phosphatidylglycerol (0.018-0.1%); whey proteins (1.5-3.5% weighed); corn oil (15-25%). Oil-in water emulsions were prepared. Emulsion stability was evaluated by autoclaving samples at 121oC for 15 min to check for oil separation. Emulsion samples were also shaken at 100 rpm for 30 min. Turbidity measurements were carried out at 500 nm for 10 min. After 10 experiments in the first cycle, optimal conditions for the preparation of stable lutein enriched dressing emulsion (no oil separation during autoclaving and mechanical shaking) were: corn oil: 20%, phosphatidylglycerol 0.018% weighed, pH: 4.5; lutein concentration: 0.015; and whey proteins concentration:2%. Lutein enriched salad dressings may be a novel health food product for all concerned about vision health in old age.
Session 45I, Nutrition: General
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