88-9 |
In vitro and in vivo inhibition of angiogenic differentiation by rice bioactive poly/peptides |
H. A. BAWADI, School of Human Ecology, Louisiana State Univ. Agricultural Center, 125 Human Ecology Bldg., Baton Rouge, LA 70803 and J. N. Losso, Dept. of Food Science, Louisiana State Univ. Agricultural Center, 111 Food Science Bldg., Baton Rouge, LA 70803-4200. Angiogenesis-the formation of new blood vessels from preexisting vascular ones- has been identified as a safe target for the prevention of the onset and /or progression of several neoplastic and non neoplastic chronic diseases. Functional foods that inhibit the angiogenic process may help delay the onset of several chronic neoplastic and non neoplastic diseases. The rationale of functional foods as anti-angiogenic compounds is that most of them are safe, effective, irreversible, and can be administered over the lifespan of an individual. The objective of this study was to evaluate the in vitro and in vivo ability of the bioactive protein rice Bowman-Birk inhibitor (rBBI) and peptides generated by the hydrolysis of rice prolamin , against stimulators of angiogenesis. We investigated the effect of rice Bowman-Birk inhibitor and rice prolamin peptides on the tube formation of human umbilical vein endothelial cells (HUVEC) on matrigel. Tube formation was inhibited by treatment with 1 mg/ml of rBBI and 40 mM of prolamin peptides both prior to plating and after plating endothelial cells on matrigel. Result of zymography showed that rBBI and prolamin peptides, at 62.5 mM and 40 mM respectively, also inhibited the activation of pro-metalloproteinase-9 (pro-MMP-9), an enzyme associated with the degradation of basement membrane leading to the progression of angiogenesis and metastasis. In vivo angiogenesis using the chick chorioallantoic membrane assay (CAM) showed that rBBI at 1 mg/ml and rice prolamine peptides at 40 mM, respectively inhibited the formation of new blood vessels on the growing chick embryo. These findings suggests that rBBI and rice prolamin peptides inhibit angiogenesis by modulating the activity of proteases associated with the angiogenic process. Bioactive compounds from rice with inhibitory activity against angiogenesis may be considered as functional foods and find applications into new food formulations.
Session 88, Nutraceuticals & Functional Foods: General
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