88-8 |
Extraction and GC/MS determination of bioactive constituents in feverfew |
C. WU1, F. Chen1, X. Wang1, and H. Y. Chung2. (1) Dept. of Food Science & Human Nutrition, Clemson Univ., 224 Poole Agricultural Ctr., Box 340370, Clemson, SC 29634-0371, (2) Biology Dept., Chinese Univ. of Hong Kong, Food & Nutritional Sciences Programme, Shatin, New Territories, Hong Kong SAR, China Medicinal herb feverfew (Tanacetum parthenium) is well known because of its prophylactic properties over migraine and arthritis. Parthenolide, a sesqiterpene lactone in feverfew, is reported to be the primary pharmacological compound for its curative properties. Another flavonoid in feverfew, Apigenin, is also reported to have anti-carcinogenic effect. The object of our study is to develop a rapid, economic and effective extraction method to obtain most biological and pharmacological compounds from feverfew. We tested two extraction methods (Soxhlet and Bar-stirring) using six solvents (acetonitrile, ethanol, methanol, hexane, dichloromethylene, and acetone) on different water content (20%, 10%, 0%). HPLC and GC-MS are used to determine quantitatively and qualitatively the bioactive constituents in feverfew. The results show that feverfew powder extracted by 80% ethanol using bar-stirring liquid extraction for 10 min has higher yield of parthenolide compared to other solvent extraction methods. The bioactive function and stability of this extract are under investigation. This extraction method may be applied to replace the current commercial method to extract more nutraceuticals in feverfew.
Session 88, Nutraceuticals & Functional Foods: General
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