14E-5 |
Antibacterial activity of panduartin A isolated from Kaempferia pandurata against oral microorganisms |
J. Y. CHUNG1, K. M. Park1, J. E. Kim2, N. I. Baek3, and J. K. Hwang4. (1) Department of Biomaterials Science and Engineering, Yonsei University, B118CA Yonsei Engineering Complex, 134 Shinchon-dong Sudaemun-gu, Seoul, 151-142, South Korea, (2) Bioproducts Research Center, Yonsei University, B118CA Yonsei Engineering Research Complex, 134 Shinchon-dong Sudaemun-gu, Seoul, 120-749, South Korea, (3) Dept. of Life Sciences, Kyung Hee University, Yong-In, South Korea, (4) Department of Biotechnology & Bioproducts Research Center, Yonsei University, B138A Yonsei Engineering Complex,134 Shinchon-dong Sudaemun-gu, Seoul, 120-749, South Korea Periodontitis has a massive impact on mankind with dental plaque. If periodontitis progresses unchecked, the results conclude periodontal pocket formation, loosening teeth, and subsequent tooth loss, and oral malodor occurrence. In recent years, many studies have been focused on the role of specific bacteria for initiation and progression of periodontal diseases as well as response to therapy. Our preliminary antibacterial screening for tropical plants showed that the extract Kaempferia pandurata, widely cultivated in Thailand and Indonesia for the spicy flavour, possessed strong inhibitory activity against Porphyromonas gingivalis. The purpose of this study was to identify and characterize the active compound isolated from Kaempferia pandurata. The methanol extract of Kaempferia pandurata was fractionated with ethyl acetate, butanol and water, in which the ethyl acetate fraction exhibited comparatively higher inhibition than other fractions. Silica gel column chromatography and recycling prep-HPLC were performed to give a single active compound and its structure was analyzed by 13C-NMR, 1H-NMR, and FAB-MS. The purified compound from Kaempferia pandurata was identified as panduratin A (C26H30O4), a cyclohexenyl chalcone derivative. Panduratin A exhibited significantly low MIC value (4 งถ/mL) against P. gingivalis. Panduratin A also possessed preferential activity against prevotella intermedia and P. loescheii. In addition, panduratin A exhibited fast bactericidal activity against Streptococcu mutans in a minute at the concentration of 20 งถ/mL. MIC value(4 งถ/mL) of panduratin A against S. mutans was much lower than 1,000 งถ/mL of eucalyptol, 125 งถ/mL of green tea extract and thymol, and 8 งถ/mL of sanguinarine. Significant damage on cell wall and membrane by panduratin A was observed by transmission electron microscopy (TEM). In summary, the results strongly suggest that the specific activity of panduratin A against oral pathogens, fast-effectiveness, and cell safety can be developed as a natural antibacterial agent for functional foods or oral care products
Session 14E, Nutraceuticals & Functional Foods: General I
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