14E-37 |
Suppression of cyclooxygenase-2 and inducible nitric oxide synthase by panduratin A in RAW 264.7 macrophages |
J. M. YUN1, H. J. Kwon1, S. H. Lee2, and J. K. Hwang2. (1) Department of Food & Nutrition, Seoul National University, San 56-1, Shillim-dong Kwanak-gu, Seoul, 151-742, South Korea, (2) Department of Biotechnology & Bioproduct Research Center, Yonsei University, B118CA Yonsei Engineering Complex, 134 Shinchon-dong Sudaemun-gu, Seoul, 120-749, South Korea (1) Panduratin A is a polyphenolic compound isolated from Kaempferia pandurata (Zingiberaceae family), which has been used as an edible medicinal plant for the treatment of inflammation, cancer, dysentery, colic disorder, tussive symptom, etc. However little studies have been reported for anti-inflammatory activity of panduratin A. Prostaglandins biosynthesis and nitric oxide production are closely involved in the process of inflammation and carcinogenesis. (2) The purpose of this research was to examine the effect of panduratin A on the induction of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in RAW 264.7 macrophages activated with lipopolysaccharide. (3) The methanol extract of Kaempferia pandurata was fractionated with ethyl acetate, butanol and water. Silica gel column chromatography and recycling prep-HPLC were performed to obtain panduratin A and its structure was analyzed by 13C-NMR, 1H-NMR and FAB-MS. Nitrite and prostaglandin E2 production were measured by the Griess assay using a enzyme immunoassay kit. Expression of the iNOS and COX-2 gene were assessed by Western blot assay and reverse transcriptase polymerase chain reaction (RT-PCR). Activation of NF-リB was determined by gel mobility shift assay and Western blot analysis of IリB-メ. (4) Panduratin A was a potent inhibitor of nitric oxide and prostaglandin E2 production without an appreciable cytotoxic effect on Raw 264.7 macrophages and this effect was a dose-dependent manner. Western analysis and reverse transcriptase polymerase chain reaction showed that panduratin A decreased the levels of expression and mRNA in a dose-dependent manner. Panduratin A inhibited the activation and translocation of nuclear factor リB (NF-リB) to the nuclear by suppressing the degradation of its inhibitory protein IリB-メ in the cytoplasm. (5) This study suggests that modulation of iNOS and COX-2 by panduratin A may be important in the prevention of inflammation and carcinogenesis.
Session 14E, Nutraceuticals & Functional Foods: General I
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