45I-12 |
Inhibitory effect of aspirin on azoxymethane-induced preneoplastic colonic aberrant crypts In Fisher 344 male rats is enhanced by prebiotic-synergy1 |
M. VERGHESE1, L. T. Walker1, I. A. Bonsi, J. E. W. Richardson1, L. Shackelford, C. B. Chawan1, and J. V. Loo4. (1) Dept. of Food & Animal Sciences, Alabama A&M Univ., Nutrition & Carcinogenesis Lab., PO Box 1628, Normal, AL 35762-1628, (2) ORAFTI, Aandorenstraat 1, Tienen, Belgium Colon cancer is one of the leading causes of cancer mortality and morbidity in the US. The objective of this study was to test the possible combinational effects of Raftilose ® Synergy1 (HP Inulin + Oligofructose) and Aspirin (a Non Steroidal Anti-inflammatory Drug-NSAID) on azoxymethane (AOM)-induced aberrant crypt foci (ACF). Following an acclimatization period of 1 wk, 45 male weanling rats were divided into 4 groups and fed AIN93G (Control-C) and 3 experimental diets that contained C +10% synergy1, C + 200ppm Aspirin, C + 10% synergy1 + 200ppm Aspirin, respectively. All the rats received 16mg/kg body weight of AOM dissolved in saline s/c at 7 and 8wk of age. The rats continued to receive the assigned diets until sacrifice by CO2 asphyxiation at 17 wk of age. There was a significant (p<0.05) increase in cecal weight and a decrease in cecal pH from 7.49 in control group to 6.04 and 6.90, in the groups fed synergy1, synergy1+ Aspirin, respectively. Dietary administration of synergy1 and aspirin significantly (p<0.05) suppressed induction of colonic ACF. The percent reductions in ACF in the experimental groups compared to the control were 52.8 (C + 10% synergy1), 59.1(C + 200ppm Aspirin), 70.3 (C + 10% synergy1 + 200ppm Aspirin), respectively. The results of this study indicate that the prebiotic synergy1, when given in combination with Aspirin, a NSAIDs, had a significantly (p<0.05) higher effect in reducing AOM-induced ACF formation than either one given singly.
Session 45I, Nutrition: General
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