42-2 |
PCR detection of bovine residues in meat and bone meal and other commercial ingredients of animal feed |
S. H. KIM1, C. I. Wei2, and H. An1. (1) Dept. of Nutrition & Food Science, Auburn Univ., 328 Spidle Hall, Auburn, AL 36849-5605, (2) Nutritional Sciences, Oklahoma State University, Stillwater, OK 74078 Bovine spongiform encephalopathy (BSE) has been a tremendous threat to both animal and human health since the first case of BSE was reported in the U.K. in 1986. It is believed that the rendered feed ingredient, meat and bone meal (MBM), contaminated with a transmissible spongiform encephalopathy (TSE) agent served as the common source of infection. In 1988, the EU banned the inclusion of ruminant derived protein in animal feed. The U.S. Food Drug and Administration also introduced the feed ban to prevent the spread of BSE throughout animal feed in 1997. The objective of this study was to investigate the presence bovine MBM in commercial ingredients for animal feed. The PCR assay using mitochondrial gene targeted primers was used for the detection of bovine DNA in bovine, deer, and porcine MBMs and 30 different ingredients used for animal feed. Two different DNA extraction methods (silica bead and column) were applied for a better detection of bovine DNA in various samples. Amplified PCR products were analyzed by comparing DNA ladder on 2% agarose gel The PCR amplicon (271 bp) was only observed in bovine MBM samples prepared by both silica bead and column DNA extraction methods. However, when the DNA was extracted from other bovine samples such as milk protein and bovine serum albumin, the silica bead method showed the better result in PCR amplification than column extraction method. The PCR assay showed negative results with non-bovine, such as fish meal, feather meal, soybean meal, wheat, whey, soy hulls, and corn. The PCR assay showed the specificity in detecting bovine DNA in different species of MBM samples, and the silica based extraction method was more effective for DNA extraction from various types of samples. The contamination of bovine MBM was not detected in the non-bovine commercial ingredients tested.
Session 42, Food Chemistry: Proteins II
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