14E-36 |
Supercritical CO2 extraction of lutein from Marigold flower |
H. R. ALIEE1, R. Tsao2, R. X. Yang2, M. F. Marcone1, and Y. Kakuda1. (1) Dept. of Food Science, Univ. of Guelph, Food Science Bldg., Guelph, ON N1G 2W1, Canada, (2) Food Research Program, Agriculture & Agri-Food Canada, 93 Stone Rd. W., Guelph, ON N1G 5C9, Canada Lutein is a carotenoid found to be associated with reduced risks of age-related macular degeneration of the human eye, and lower incidence of cardiovascular diseases due to its antioxidant properties. It is conventionally extracted from dried marigold flowers with organic solvents such as hexane. The objective of this study is to obtain optimized conditions for the parameters in the supercritical CO2 extraction technology, and to examine the potential of using this as an environmentally benign alternative to the solvent extraction. Hewlett-Packard 7680T supercritical fluid extractor with octadecyl functionalized silica trap was used. The extracted Oleoresin were analyzed by HPLC. It was found that increase in pressure directly resulted in increased extraction yield of lutein, whereas increase in static and dynamic extraction times did not show significant difference in extraction yield. Supercritical CO2 Extraction demonstrated to be an effective method for extraction of lutein from marigold. This technology can be used as an alternative technique to organic solvent extraction process.
Session 14E, Nutraceuticals & Functional Foods: General I
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